Isothermal amplification of nucleic acids is a biochemical reaction that rapidly and efficiently accumulates nucleic acid sequences at a constant temperature. Isothermal amplification techniques have been developed as alternatives to polymerase chain reaction (PCR), which requires sophisticated thermocycler equipment. Because of their simplicity, these techniques are particularly useful in diagnostic applications and have been used for biosensing targets such as DNA, RNA, cells, proteins, small molecules, and ions. Isothermal amplification reactions usually require the concerted actions of multiple protein components and nucleic acid sequence, such as DNA binding proteins, recombinase and polymerase.
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