Overview:

The 2019-nCoV High Sensitivity S1 Protein ELISA Kit v2.0 is a sandwich enzyme-linked immunosorbent assay for the quantitative measurement of 2019-nCoV Coronavirus Spike protein. The S1 Protein ELISA employs a capture anti-S1 protein antibody coated on a 96-well plate. Calibrators and samples are pipetted into the wells; the target protein in the calibrators and samples binds to the immobilized capture antibody. The wells are washed and the HRP-labeled Detection antibody is then added. After washing away the unbound HRP-antibodies, a colorimetric substrate solution (TMB) is pipetted to the wells. The absorbance of the color at 450 nm is measured. The intensity of color development in the wells is proportional to the amount of target protein bound.

Components:

References:

1.Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89.

2. Xiao X, et al: The SARS-CoV S glycoprotein. Cell Mol Life Sci. 2004, 61 (19-20): 2428-30.

3. Lan J, et al: Crystal structure of the 2019-nCoV Spike receptor-binding domain bound with the ACE2 receptor. bioRxiv. doi: https://doi.org/10.1101/2020.02.19.956235.