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2019-nCoV Neutralization ELISA Kit

Catalog No. C19SD-N887


$620.00
96 Reactions Per Kit

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Overview:

The 2019-nCoV Neutralization ELISA Kit is an enzyme-linked immunosorbent assay for the detection of antibodies or other proteins (molecular) that can block the binding of SARS-CoV-2 S1 protein and human ACE2 in serum or other biological samples. Based on indirect ELISA principles, the 2019- nCoV Neutralization ELISA employs recombinant human ACE2 protein coated on a 96-well plate. Controls and samples (with necessary dilution) are mixed with 2019-nCOV S1 protein RBD-HRP and then pipetted into the wells; the target neutralizing antibodies or proteins in the controls and samples will partially or completely block binding of the S1 protein RBD-HRP to the immobilized ACE2 protein. After washing away the unbound 2019-nCOV S1 protein RBD-HRP, a colorimetric substrate solution (TMB) is pipetted to the wells. The absorbance of the color at 450 nm is measured. The intensity of color development in the wells is proportional to the amount of target protein bound.


Components:

SignalChem Cat# Component Name Size Storage
C19SD-G241DH2019-nCoV Spike protein RBD, HRP Conjugate8 ml x 1 vialsStore kit and contents at 4oC for up to 6 months. Store unused multi well strips in the provided resealable bag and use within one month after opening.
CP02-A51C2ACE2 (19-740) Coated Plate1 plate
E01-09nCoV ELISA Sample Dilution Buffer10 ml x 1 vials
SS01-09Stop Solution6 ml x 1 vials
C19N-NEGNAb Negative Control150 ul x 1 vials
C19N-POSNAb Positive Control150 ul x 1 vials
WB20-09Wash Buffer, 20X15 ml x 1 vials
CS02-506One-Step TMB Substrate10 ml x 1 vials

References:



1. Zhou P, et al: A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature. 2020, 579:270-89.

2. Xiao X, et al: The SARS-CoV S glycoprotein. Cell Mol Life Sci. 2004, 61 (19-20): 2428-30.

3. Long Q, et al: Antibody responses to SARS-CoV-2 in patients with COVID-19 Quan-Xin Long. Nature Medicine. 2020, 29 April.

4. Ma H, et al: Serum IgA, IgM, and IgG responses in COVID-19. Cell Mol Immunol. 2020, 17: 773-775.



Representative Data:

In order to evaluate the presence of Neutralizing antibodies or proteins in a biological sample, the O.D. value of the sample must be compared to the values obtained for the positive and negative controls.


Related Products:

Name Catalog Number Species Tag Expression System Sequence Genbank Number
2019-nCoV Spike protein RBD, HIS TagC19SD-G241HSARS-CoV-2HISCHO cells319-541MN908947
2019-nCoV Spike protein RBD, Fc TagC19SD-G241FSARS-CoV-2FcCHO cells319-541MN908947
2019-nCoV Spike protein S1, HIS TagC19S1-G241HSARS-CoV-2HISCHO cells16-685MN908947
2019-nCoV Spike protein S1, Fc TagC19S1-G241FSARS-CoV-2FcCHO cells16-685MN908947
2019-nCoV Spike protein S2, Fc TagC19S2-G241FSARS-CoV-2FcCHO cells686-1212MN908947
Anti 2019-nCoV Spike Protein hIgG Antibody, HRP conjugated C19S1-60DHHuman, IgG1CHO cells
Anti 2019-nCoV Spike Protein mIgG AntibodyC19S1-61MHHuman Fab – Mouse IgG FcCHO cells
Anti 2019-nCoV Spike Protein hIgA AntibodyC19S1-A60HHuman, IgACHO cells
Anti 2019-nCoV N-Protein hIgG Antibody, HRP conjugatedC19NP-60DHHuman, IgG1CHO cells
2019-nCoV S1 Protein ELISA KitC19SD-876
2019-nCoV S1 Human IgG-IgA-IgM ELISA KitC19S1-P877
2019-nCoV High Sensitivity S1 Protein ELISA KitC19S1-876


Assay Workflow:


Technical Manual:


CoA Sheet:


SDS:



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