Enzymes, Signaling Proteins, Extracellular Ligands, Signaling Reagents, Antibodies
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Frequently Asked Questions

Technical Questions
For all of our products we run SDS-PAGE gel with a titration of the enzyme, followed by the densitometry measurement to determine the purity.
To request the sequence information for the protein of interest please contact us.
An enzyme titration curve and calculated specific activity are included in the data sheet of each active enzyme. It is recommend that the researchers perform a serial dilution of an enzyme product to determine the optimal amount to use in their own assay systems.
The concentration of each of our products is calculated from a titration on SDS-PAGE gel with a standard curve of the Bovine serum albumin (BSA) or the Bovine gamma globulin (BGG).
Yes, we may provide enzymes at a higher concentration. Please contact us to obtain more detailed information.
Yes, we can supply our protein in a customized buffer formulation. Please contact us to submit your request.
SignalChem’s active enzymes are produced using specific treatment or conditions to achieve optimal activity in vitro, while unactive proteins are generally produced without any stimulation or from prokaryotic systems so the enzymes only show minimal basal activity. Active enzymes can be directly used in biochemical assays for compound screening or enzymatic studies. Unactive proteins can be used as substrates, negative controls, or to study activation of the particular enzymes in vitro.
SignalChem’s active enzymes are activated using specific protocols during the process of expression and production to achieve optimal post-translation modification and in vitro activity. The treatment methods may vary for different enzymes depending on their functional roles.
Yes, we do customize the proteins with other tags (e.g. FLAG, Fc). Please contact us for the detailed information.
Yes, we can customize the protein as per your requirements. Please contact us to submit your query.
Please contact us to obtain the details for tag cleavage site.
Expression system is chosen based on the function and application of each product.
SignalChem’s active enzymes are validated for in vitro activity assays. We offer a wide range of custom services including additional purification steps, buffer exchange, and tag modification to fulfill the requirements for other applications such as protein crystallography. Please contact us find more information about this service.
SignalChem’s active enzymes and recombinant proteins both may work in binding assays. We recommend clients to test the compatibility of a product in their assay system. Contact us to request a free sample.
SignalChem offers Profiling Services for enzymatic studies including Km determination. Please click here to find more information about this service.
Generally active kinases are phosphorylated on certain sites in their catalytic domain. However, the number of total phosphorylation sites from each active kinase may vary. We do not perform any test to probe for specific phosphorylation in our kinases.
Phosphorylation of a certain residue can be determined by Western Blot or ELISA using phospho-specific antibodies.
Shipping Questions
All orders to be shipped within North America, which are received by 10:00 AM PST Monday to Wednesday, will usually be shipped out on the same day. International orders are shipped on Mondays and Fridays.
Freight charges vary depending on the final destination. SignalChem is not responsible for any fees that may be incurred by your order such as brokerage costs, duties and taxes. Please contact us to obtain an estimate.
Yes. We also ship with UPS and World Courier.
To ensure the quality of our product, SignalChem does not recommend customers to use products that arrived without dry ice left in the package. A replacement may be sent under specific circumstances. Please contact us to request a replacement.
The shelf life of a product is indicated on the datasheet. SignalChem guarantees that all of its products meet the specific requirements set forth within the lot specific technical data sheets (TDS). Should you have a technical issue with one of our products, please contact us with your specific query.
If an incorrect product is ordered by the customer, SignalChem would accept returns under these circumstances:

• The product is in its original packaging and stored at the recommended condition
• A 30% restocking fee will be applied to absorb our cost of delivering the products.
• Customer will be responsible for the return of freight charges.

If it is determined, solely by SignalChem, that one of our products has failed to conform to the specifications set forth in the technical datasheet, we will gladly replace the product free of charge or issue a credit for the total purchase price of the non-conforming product.

Still have questions?

Please feel free to contact us via our Online Support Form or via Phone or Email
Tel: 1-866-6-KINASE (1-866-954-6273) or 1-604-232-4600
Email: support@signalchem.com

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