Recombinant full length human UBE2F was expressed in E. coli cells using an N-terminal His tag.
Catalog No. U220-30H
Catalog No. | Pack Size | Price (USD) | |
---|---|---|---|
U220-30H-20 | 20 ug | $105 | |
U220-30H-50 | 50 ug | $160 | |
U220-30H-BULK | BULK | Contact Us |
Overview:
UBE2F or ubiquitin-conjugating enzyme E2F is part of the ubiquitin network for protein degradation and is involved in protein neddylation. UBE2F has been shown to function as a NEDD8-conjugating enzyme in vitro and in vivo (1). Covalent attachment of the ubiquitin-like protein NEDD8 to a conserved C-terminal domain (ctd) lysine stimulates Cullin Ring ligase (CRL) ubiquitination activity and prevents binding of the inhibitor CAND1 (2).
Gene Aliases:
NCE2
Genbank Number:
References:
1. Huang, D T. et al: E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification. Mol Cell. 2009 Feb 27;33(4):483-95. 2. Boh, B K. et al: Neddylation-induced conformational control regulates cullin RING ligase activity in vivo. J Mol Biol. 2011 Jun 3;409(2):136-45.
Purity:
Sample Purity Data. For specific information on a given lot, see related technical data sheet.
Storage, Stability and Shipping:
Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Molecular Weight:
~24 kDa
There are no related publications available for this product.
Cancer, Cell Cycle, Neurobiology
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